Dietary fructo-oligosaccharide supplementation modulated the neurological, cardiovascular function and antioxidant level in D-galactose-treated Balb/c mice

• Main Authors: Meng-Jun, 洪孟君
• Other Authors: 陳曉鈴
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/76096094763453367819

碩士 === 中山醫學大學 === 營養學系碩士班 === 99 === Objectives: This experimental study long-term injection of D-galactose-induced Balb/ c mice associated with aging syndrome, including brain β-amyloid formation, learning and memory function, regulation of cardiovascular function and antioxidant status; and dietary supplement of fructo-oligosaccharide (FOS) or vitamin E can delay the aging process. Materials and Methods：The twelve-week-old male Balb / c mice were divided into control group, D-galactose group, D-galactose low-FOS group (2.5% w/w), D-galactose high-FOS group ( 5% w/w) and D-galactose vitamin E group (0.2% w/w), diet and subcutaneous injection of saline (control group) or 10% D-galactose saline (1.2 g / kg BW) continuous involvement of 49 days. Another natural aging group of feed intake control diet, but not vaccinated, 64 weeks old great sacrifice. Experiment of end for 5-7 days to measure water maze (learning and memory function) and before 3 days measure ECG and blood pressure. Fasting bloods were collected from the heart and perfusion with ice-cold saline, after collection of brain, heart, liver, and measured left ventricular thickness. The plasma and liver were stored at -80 ℃ until subsequent analysis for blood lipids and antioxidant molecules. The brain tissue dehydrated of sucrose solution, the frozen sections and β-amyloid immunostaining. Result: Natural aging group and D-galactose group have similar neurological degenerative of aging, such as learning and memory degeneration, brain beta-amyloid accumulation increased. Diet supplement FOS or vitamin E can delay the degenerative neurological conditions. In the cardiovascular function, natural aging group and D-galactose group the heart rate and left ventricular thickness was significantly higher than the control group, and systolic blood pressure, diastolic blood pressure and mean arterial pressure were increase. Natural aging group and D-galactose group the QRS and QTc intervals of ECG have extended, but added FOS or vitamin E can regulate blood pressure and heart function. Natural aging group and D-galactose group the plasma total cholesterol and triglycerides were significantly higher than the control group, supplement of FOS or vitamin E can decline blood lipids. In antioxidants, the plasma vitamin C concentration in each group no significant differences, but the natural aging group and D-galactose group have lower concentration, but supplement FOS or vitamin E can increase concentration. The plasma vitamin E of the control group was significantly higher than the natural aging group and D-galactose group whereas supplement of FOS or vitamin E can enhance the concentration. In the liver total glutathione (GSH), the control group than natural aging group and D-galactose group of high-trend, but no significant differences. The GSH level of the control group was significantly higher than those of the natural aging group and D-galactose group. FOS or vitamin E can increase liver total GSH and the reduced GSH content. The GSSG/GSH ratio of the natural aging group and D-galactose group was significantly higher than the control group, supplement FOS or vitamin E can reduce oxidative stress. Conclusion: This study indicated that D-galactose injection accelerated decline of brain function and cardiovascular function, blood and liver vitamin E depletion. The dietary supplement of FOS can delay D-galactose-induced brain lesions and cardiovascular function, and maintain plasma and liver antioxidant of molecular concentration.