Comparison of antioxidative and hepatoprotective activities of various buckwheat extracts

• Main Authors: Ya-hsien, 許亞嫺
• Other Authors: 蔡惠燕
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/80802554944285758776

碩士 === 中山醫學大學 === 應用化學系碩士班 === 99 === Buckwheat used to be a product for natural disaster relief, but is now an industrial crop with high economic value. Bcckwheat is recognized as a valuable source of flavonoids. Flavonoids such as rutin and quercetin, are anti-oxidants known for reducing cardiovascular diseases and radical scavenging activity. Rutin and quercetin are more antioxidative effect than that of vitamin C and vitamin E. The phytochemical content are varied for variety of breads and cultivation. Therefore, this study compared the liver protection activity of extracts from different breeds, cultivation of buckwheat. By treating hepatocytes with t-BHP that induces the oxidative damage of in-vitro hepatocytes, the protective activity of buckwheat on the hepatocytes was determined. All the extracts demonstrated protective activity and reduced the extent of t-BHP damage on the hepatocytes. Total antioxidative activity of the extracts was measured by FRAP assay. As the concentration of the ethanol of the extracting solvents increased, the extract displayed better antioxidative effect. The rutin and quercetin of the extracts were analyzed by HPLC-UV. The results indicate that the rutin content of tartary buckwheat (Fagopyrum tataricum Gaertn) is higher than that of common buckwheat (Fagopyrium esculentum Moench), while the rutin was found in the groat and hull of buckwheat cultivated in Erlin and Mongolia. The rutin content is higher than the quercetin content in the hull of common buckwheat, and the rutin and quercetin content in the groat of the tartary buckwheat are higher than those of the hull of the tartary buckwheat. These results are consistent with those reported in the literatures. However, there are many substances not been identified in present work. These unidentified substances may cause cytotoxicity and set off the antioxidative effect of other components. Some extracts containing excessive flavonoids such as rutin and quercetin, may case over dose problems. The extracts of groat of common buckwheat cultivated in Taiwan show better protective activity compared to those imported from Mongolia. The results indicate that the locally cultivated buckwheat has higher added value.