The preventive effect of Solanum nigrum water extract in Diclofenac-induced rat gastric mucosal damage

• Main Authors: Jia-Yuan, 黃佳元
• Other Authors: 許振東
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/16207916031912188712

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 99 === Diclofenac is a kind of non-steroid anti-inflammation drug. Under oral administration, it has the side effect of gastric mucosal damage. Some literature indicates that Solanum nigrum has functions of anti-inflammatory and anti-oxidation. Therefore, we evaluate whether the Solanum nigrum water extract (SNE) can prevent the gastric mucosal damage caused by Diclofenac. Different dosages of Solanum nigrum water extract, and proton pump inhibitor (Esomeprazole, 500 μg/kg) as preventive medicine for the protection reference are given to the Wistar rat for experiment, then oral administration of Diclofenac (100 mg/kg) inducing gastric mucosal damage. By analysis of white blood cell count, activity of antioxidant enzyme glutathione-S-transferase (GST) in red blood cell, as well as the concentration of gastric mucosal lipid peroxide thiobarbituric acid reactive substances (TBARS) and the antioxidant enzyme glutathione (GSH), as well as histological examination of stomach. The result of our study showed that evaluate the protection for stomach provided by Solanum nigrum water extract. Compared with experiment group and Diclofenac test group, a remarkable promotion on activity of GSTs and the concentration of GSH in experiment group is noted. Moreover, it presents dose-dependent to the Solanum nigrum water extract. Obvious reductions on white blood cell count and the concentration of TBARS in gastric mucosa are also found and present dose-dependent to Solanum nigrum water extract. The pathology examination reveals that treatment of Solanum nigrum water extract may prevent the gastric mucosal damage. To sum up, the Solanum nigrum water extract can prevent the gastric mucosal damage caused by Diclofenac. The results maybe due to antioxidation and the ability of free radical scavenging of Solanum nigrum water extract. The Solanum nigrum water extract surely can be utilized as protecting agent of gastric mucosa.