| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 99 === The Perilla frutescens Britt. (PF), a traditional Chinese medicinal herb, has been used in China for centuries to treat various diseases including bacterial and fungal infections and allergic diseases.House dust mites are well-known as a source of indoor aeroallergens and for causing allergic airway diseases. In this study, we investigated the anti-allergic effect of methanol extract of PF leaf (PFE) on Dermatophagoides pteronyssinus 2 (DP2)-stimulated bronchial epithelial cell line (BEAS-2B). Pro-inflammatory cytokines mRNA expression was analyzed by RT-PCR and quantitative real-time PCR. Activation of kinase cascades were investigated by immunoblot. Our results revealed that PFE significantly reduced the DP2-induced mRNA expression of IL-4, IL-5, IL-6, IL-8, IL-13, MCP-1 and GM-CSF in a dose-dependent manner. A mechanism based kinase cascades analysis showed that PFE suppressed the DP2- induced phosphorylation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinase1/2 (ERK1/2) , P38 MAPK(p38) and c-Jun N-terminal kinase (JNK).Additionally,we also found that PFE inhibited the translocation of NF-κB/p65 into nucleus which may result from the suppression of IκBα degradation. Taken together, these findings indicate that PFE significantly alleviates DP2-induced inflammatory responses, which may attribute to suppression of MAPK signaling and nuclear of NF-κB, suggesting that PFE should be beneficial to treatment of allergen-induce inflammation.
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