| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 99 === Malignancy of human disease is currently one of the most difficult to overcome, the major methods of cancer therapy is surgical treatment. Many studies have indicated that Chinese herbal medicine have anti-oxidative, anti-inflammatory and anti-tumor effects, the mainly function of Chinese herbal medicine is to promote cancer cells death by induce apoptosis and low side effects for human. Many studies have also shown that phenolic compound extracts from plants have anti-cancer function. The polyphenols contained in mulberry leaves has been shown to inhibit cancer cell proliferation, invasion and metastasis. In this study, we demonstrated what the mulberry leaf polyphenols extract (MLPE) expressed cytotoxic effects to many cancer cell lines. Among them, human hepatocellular carcinoma (HCC) cell, HepG2, was the most susceptible to MLPE. We also used MTT assay to evaluate the cytotoxicity on the other HCC cell (Hep3B) and normal liver cell (Chang liver cell). The results show that HCC cells were more susceptible to MLPE than normal liver cell. Next, we use cell model to explore the anti-cancer effects of MLPE in HCC cells. And we observed the effects of MLPE in inducing apoptosis in Hep3B by measuring the nuclear condensation, DNA fragmentation and increased the subG1 phase ratio in cell cycle. This effect of MLPE in Hep3B might be mediated via increase the expression the Fas and cleaved-caspase 3, 8, 9 proteins. And we also observed the effects of MLPE in inducing autophagy in HepG2 by measuring the Acidic vesucular organelles (AVO) formation. This effect of MLPE in HepG2 cells might be mediated via inhibit the expression PI3K/Akt and then increase the expression the Beclin 1 and LC 3-II proteins. Furthermore, we think the different effects of MLPE in inducing HCC cell to apoptosis or autophagy is due to P53. Several researches indicated Fatty acid synthase (FASN) as molecular targets for cancer therapy. We also observed FASN inhibition by MLPE. This relationship between FASN formation and the HCC remains to be further research. The antitumor efficacy of MLPE was confirmed in HCC cell lines. Our data indicate MLPE could play an active role in mediating the apoptosis or autophagy of HCC cells and might be potential drugs for antitumor therapy and functional health food.
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