| Summary: | 碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 99 === Staphylococcus aureus is the pathogen of nosocomial infections and the etiologic agent of a wide range of diseases including endocarditis, osteomyelitis, toxic shock syndrome, food poisoning, and skin infections. S. aureus to form multilayered biofilms on host tissue and other surfaces, exhibits increasing resistance to antibiotics. Polygonum cuspidatum Sieb. et Zucc. (P. cuspidatum) has been used as a folk medicine for inflammatory disease, hepatitis and tumor in East Asia. The investigation is to explore the effect of the ethyl acetate subfraction from Polygonum cuspidatum root and emodin on S. aureus biofilm formation. Results showed that the ethyl acetate subfraction F3 from Polygonum cuspidatum root and emodin inhibited the biofilm formation of the S. aureus SA 113 and methicillin-resistant S. aureus MRSA16 and MRSA17, the concentration that inhibited formation of 50% biofims (IB50) were 8.1μg/ml, 7.2 μg/ml and 6.6 μg/ml, 4.2 μg/ml, 5.1 μg/ml and 4.5 μg/ml, respectively. Besides, we found that 6.25 μg/ml of F3 and emodin inhibited polysaccharide intercellular adhesion (PIA) biosynthesis (62% and 65%, respectively) and interrupted the initial attachment stage of biofilm formation. Reverase-transcriptase-PCR showed that the transcriptional level of icaA, which encodes essential enzyme for PIA biosynthesis, was decreased after the treatment with 6.25 μg/ml of F3. These results suggested that the ethyl acetate subfraction F3 from Polygonum cuspidatum root inhibited the biofilm formation of the S. aureus by inhibiting initial attachment stage and PIA synthesis. Furthermore, Emodin is a main compound involved in F3 inhibiting biofilm formation. This investigation indicated a potential application for Polygonum cuspidatum as an adjuvant therapeutic agent for the prevention of biofilm-related infections.
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