| Summary: | 碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 99 === Ultraviolet A (UVA) is a main cause to evoke photoaging by increasing free radical production and collagen damage. Lycopene, one member of carotenoids families, is a nature liposoluble red pigment. The related studies suggest that lycopene has effective on antioxidation, recent studies had found that the influences of lycopene on skin protection, such as the effect of lycopene on expressions of collagen and UVA-induced injury. However, there is less attention to investigate the influences of lycopene-like from transformant Rhodobacter sphaeroide on skin protection. The sources of lycopene can be obtained from fruits and vegetables. Additionally, many reports indicated that lycopene could be also produced from some photosynthetic bacteria, but the selling price of lycopene is still expensive. The present study utilizes the characteristic of transformed Rhodobacter sphaeroide that can be produced on large scale to obtain not only lycopene-like smoothly but also decrease the cost. We speculate that lycopene-like may be worth to researching the new efficiency in application of skin protective field, especially in the effect of lycopene on expressions of collagen and UVA-induced injury. The effects of lycopene-like on the cell viability of human fibroblast (Hs68 cells) were evaluated by method of MTT assay. We observe the influence of lycopene-like on type I procollagen and matrix metalloproteinase (MMP-1 and MMP-2) by western blot analysis and gelatin zymography. On the other hand, we also evaluated expression of type I procollagen and MMP-1 in Hs68 cells incubated with lycopene-like or lycopene after pretreatment with UVA radiation. It was found that lycopene-like had only weak effects on cell cytotoxicity at 20 ?嵱, and the effect was equivalent to the effect of 0.1 ?嵱 lycopene (84?b3% vs. 68?b2%; 1 μM lycopene-like vs. 1 μM lycopene). Incubation with lycopene-like led to dose-dependent increased on the effects of type I procollagen. Effect on UVA-induced expression of MMP-1 and MMP-2 were significantly reduced on incubation with lycopene-like. Our findings obtain that the lycopene-like may ameliorate the UVA irradiation-induced type I procollagen decrease via mediated with the MMP-1 expression, and thereby lead to increase the collagen expression. Therefore, lycopene-like from transformant Rhodobacter sphaeroide may have great potential to further develop products and apply on skin anti-aging and anti-ultraviolet-induced injury efficacies.
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