Comparison of antioxidative constituents between Taiwan andChinese Chrysanthemum morifolium Ramat

• Main Authors: Yi-Hsiu Hou, 侯伊秀
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/wu296e

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 99 === Chrysanthemum morifolium Ramat (Chrysanthemum) belongs to the Asteraceae family. According to the color of their flowers, there are two kinds of chrysanthemum, including white and yellow chrysanthemum. Chrysanthemum has been used to treat headaches and clear the liver and eyes. Modern studies had proved that chrysanthemum have reducing cholesterol, anti-inflammation and anti-oxidantive activities. There are four chrysanthemum in Taiwan market, including white and yellow of native cultivation and Chinese chrysanthemum that were collected and extracted with H2O. Four water extracts namely, Taiwan yellow (TY), Taiwan white (TW), Chinese yellow (CY), Chinese white (CW) chrysanthemum water extracts were respectively obtained. The purpose of this study was to compare the difference of antioxdant activities and constituents between four water extracts by DPPH radical scavenging ability, Trolox equivalent antioxidant capacity, and total phenol contents. The results revealed that TY had the most effective DPPH radical scavenging ability and Trolox equivalent antioxidant capacity. CY had the most amount of total phenol contents and TY was secondary. Further to understand the constituents of four water extracts, we chose six marker components, including chlorogenic acid, rutin, luteolin, IV kaempferol, quercetin and kaempferol-3-O-β-D-glucoside, were quantitative analysis by HPLC for them. The results revealed the largest amount of chlorogenic acid and rutin were in CW, 3.36 and 7.63 mg/g respectively, the largest amount of kaempferol-3-O-β-D-glucoside (4.22 mg/g) was in CW and luteolin just found in CW (3.01 mg/g). Quercetin, kaempferol were not found in four chrysanthemum water extracts.