| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 99 === Chrysanthemum morifolium Ramat (Chrysanthemum) belongs to the
Asteraceae family. According to the color of their flowers, there are two
kinds of chrysanthemum, including white and yellow chrysanthemum.
Chrysanthemum has been used to treat headaches and clear the liver and
eyes. Modern studies had proved that chrysanthemum have reducing
cholesterol, anti-inflammation and anti-oxidantive activities. There are
four chrysanthemum in Taiwan market, including white and yellow of
native cultivation and Chinese chrysanthemum that were collected and
extracted with H2O. Four water extracts namely, Taiwan yellow (TY),
Taiwan white (TW), Chinese yellow (CY), Chinese white (CW)
chrysanthemum water extracts were respectively obtained. The purpose
of this study was to compare the difference of antioxdant activities and
constituents between four water extracts by DPPH radical scavenging
ability, Trolox equivalent antioxidant capacity, and total phenol contents.
The results revealed that TY had the most effective DPPH radical
scavenging ability and Trolox equivalent antioxidant capacity. CY had the
most amount of total phenol contents and TY was secondary.
Further to understand the constituents of four water extracts, we chose
six marker components, including chlorogenic acid, rutin, luteolin,
IV
kaempferol, quercetin and kaempferol-3-O-β-D-glucoside, were
quantitative analysis by HPLC for them. The results revealed the largest
amount of chlorogenic acid and rutin were in CW, 3.36 and 7.63 mg/g
respectively, the largest amount of kaempferol-3-O-β-D-glucoside (4.22
mg/g) was in CW and luteolin just found in CW (3.01 mg/g). Quercetin,
kaempferol were not found in four chrysanthemum water extracts.
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