Investigation the Cardio-protective Effects and molecular mechanisms of Catgut Embedding and Electro Acupuncture Therapy in TaiChong point of Spontaneous Hypertensive Rats

• Main Authors: Yi-Li Chen, 陳怡吏
• Other Authors: 陳悅生
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/23996339902908937444

碩士 === 中國醫藥大學 === 針灸研究所碩士班 === 99 === (PART 1) BACKGROUND: Hypertension is a kind of disease which the systemic arterial blood pressure is elevated. About 90–95% of cases are termed "spontaneous hypertension", which refers to high blood pressure for no medical cause can be found. It can cause serious heart, brain and kidney dysfunction. Persistent hypertension may cause cardiac fibrosis, left ventricular hypertrophy and cardiac apoptosis, leaving shortened life expectancy. Lots of clinical experiences have shown that puncture Taichong point(LR-3)can effectively lower blood pressure. OBJECTIVE: We punctured LR-3 point to observe the effect of lowering blood pressure and to explore the molecular mechanism of acupuncture treatment for hypertension. Acupuncture treatment for hypertension has less side effect. This is the common point be used in clinic. According to the research, even using the single point of Taicong(LR-3) also can lower hyper-yang activity type hypertension well. We use SHR to make sure whether the effect of electro -acupuncture on the cardiac function of SHR has a positive impact. METHODS: A total of 12 rats with 8-weeks male spontaneously hypertensive rats (SHR)were randomized divided into the acupuncture and sham groups, with 6 animals in each group. Additional 6 male Wistar-Kyoto rats were served as the control group. Rats in the acupuncture group received electro-acupuncture at bilateral Taichong (LR 3) points. Stained with Masson trichrome to observe whether there is myocardial fibrosis.and to observe myocardial cell apoptosis by TUNEL level. The expression of SOD1, FAS, FADD, Bax, cytochromC, Gαq, eNOS, and calcineurin and BNP were detected by Western blot. RESULTS AND CONCLUSION: After treatment for three days, EAT group of blood pressure in rats that have decreased significantly, including systolic(BPs), diastolic(BPd) and mean blood pressure (BPm)were significantly lower than SHR and EAS group. At 19th day , the systolic blood pressure in EAT group was still significantly lower than SHR and EAS group. Electroacupuncture LR-3 has shown that the blood pressure was went down rapidly, but the long-term continuous stimulus, its antihypertensive effects would fade. Electroacupuncture LR-3 can reduce the damage of cardiac hypertrophy and apoptosis and fibrosis caused by hypertension. According to Western blot analysis, we found the eNOS and SOD 1 were increased. In another pathway,, the hypertrophy and apoptosis protein were decreased significantly. (PART Ⅱ ) Background: the persistent high blood pressure might cause myocardial fibrosis and left ventricular hypertrophy and apoptosis. Finally shorten the life. Objective: we learned from the previous experimental Taichong electroacupuncture is effective for reducing blood pressure in spontaneously hypertensive rats, but long-term stimulation will make the effect fade in a row. So we designed a short experimental treatment and join discussions. Methods: the 8-week-old male group randomly divided into Spontaneous hypertensive rat group (SHR), pseudo-point electroacupuncture Group (EAS), electro-acupuncture in the treatment group (EAT), pseudo-acupoint embedding Group (CES), embedding therapy group (CET), for 1 week of treatment. With the 8-week-old male WKY (Wistar-Kyoto) as a control group of rats. Treatment group we chose bilateral Taichongpoint(LR-3) for electro-acupuncture and catgut embedding, and recorded their blood pressure with tail cuff. . After sacrifice we took its heart slices, and observed H&E, Masson '' s trichrome staining with TUNEL assay. Free out of the left ventricle and extraction of proteins, by Western blot to observe the proteins of anti-apoptosis and pro-apoptosis proteins and the proteins about hypertrophy or inflammation. Results and discussion: the treatment of the third day, the blood pressure of rats with has decreased significantly, including systolic, diastolic and mean blood pressure in EAT and CET group were significantly lower than SHR and EAS group, and the blood pressure in EAT group was slightly lower than the CET group , but no significant difference between the two groups. At the treatment of the 7th day EAT blood pressure in CET Group continued to decline, and in CET group blood pressure was less than EAT group but no significant difference between the two groups. We observed from the heart tissue biopsy proofed that the electro-acupuncture or catgut embedding on LR-3 can prevent cardiac hypertrophy and apoptosis and fibrosis from hypertension. Western blot analysis was found that the SOD 1 in CET group was significantly increased and apoptotic protein and hypertrophy and apoptosis protein were decreased significantly, and SOD 1 and the induction of effect of PI3K/pPI3K in CET group were better than EAT group.