| Summary: | 碩士 === 中華科技大學 === 健康科技研究所在職專班 === 99 === Bone defect is a common indication in orthopedics clinics. In the present, bone grafting is a standard treatment to increase the bone healing if the patients are not healthy or with the larger defects. Among the bone graft biomaterials, Calcium Sulfate (CS) is a kind of resorbable calcium salt which is the earliest inorganic material used as a bone graft. Although it is well biocompatible, the dissolution rate is always too fast which cannot stay long enough to support bone regeneration effectively. The pure α or β crystallized Calcium Sulfate Hemihydrate or steam treatment (in pellet) can improve the dissolution rate slightly, but the recent developed ceramic CS is far better and is competitive to the long-dissolving Calcium Phosphate Salts. The new ceramic CS material has well been studied the formula, and a cytotoxicity test has confirmed its biocompability. Since there are more additives not been mixed with CS dihydrate ever, the aim of this study were to testify its performance on preosteoblast to ascertain the safety and effect of this formula containing these additives. In our research, we are noticed that the cell number of MC3T3-E1 cell line on the two materials were statistically identical in MTT assay test. However, the secreted extracellular matrix protein and calcium mineralization showed different affections: the collagen concentration on ceramic CS was higher than on pure CS dihydrate in both 7 days and 14 day’s samples. The alkaline phosphatase (ALP) activity and calcium concentration remained identical before 7 days cultivation, but the activity of ALP and calcium sediment in ceramic CS were statically higher than in pure CS dihydrate in 14 day’s cultivation. The results implied that the new material, ceramic CS, tend to accelerate the preosteoblast’s secretion of extracellular matrix protein. We found in this study by comparing the well-known CS dihydrate that the new ceramic CS, which contains additives, shows benefit to preosteoblast cultivation.
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