In vitro verification of polycaprolactone scaffold coated with Type II collagen and manufacture of functional scaffold carrier with pressure sensing element

• Main Authors: His Jung Chen, 陳璽鎔
• Other Authors: M. Y. Lee
• Format: Others
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/15120302859914715063

碩士 === 長庚大學 === 醫療機電工程研究所 === 99 === Articular cartilage injury is a difficult self-repairing disease so that it usually needs partial cartilage or total joint replacement for preventing osteoarthritis. Tissue engineering, could proliferate cells as a customized three-dimensional tissue or organ formed by manufactured scaffold, overcomes lacks of autograft or allograft for cartilage replacement. Tissue engineering technology, used to produce bio-absorbable artificial implant with self-extracted cells, is a feasible solution in regeneration medicine. However, the surface hydriphilicity of PCL scaffold are not good for cell attachment and proliferation. Therefore, the aim of this study was design and fabricate a PCL scaffold coated type II collagen and gelatin for surface modification using selected laser sintering technique. The another aim of this study was to fabricate a PCL scaffold imbedding pressure sensing unit to be a functional scaffold for continuously monitoring pressure on repair scaffold of cartilage. In the first part, PCL (control group), type II collagen-coated PCL (experimental group A), and gelatin-coated PCL (experimental group B) scaffolds were designed and fabricated by selected laser sintering (SLS) technique. Surface hyrophilicity, porosity, biomechanical compression tests were performed to compare between three groups. In the second part, in vitro experiments (MTT, cell viability, GAG, SEM assay) were performed to determine cell proliferation ability on PCL scaffolds with or without type II collagen-coated and gelatin-coated. The results showed the mean contact angles of control group, experimental group A, and experimental group B were 121.8° ± 1.88°, 70.8° ± 2.41°, 91.5° ± 5.83°, respectively. The mean contact angle of experimental group A was smaller than other two groups. It means that type II collagen-coated PCL scaffold has the best surface hydrophilicity. Moreover, the swelling ratio of experimental group A was higher than control and experimental group B. It demonstrated that coating type II collagen could be the proposed surface modification on PCL scaffold for improving surface hydrophilicity. The mean compressive moduli of scaffolds were 6.87 MPa, 7.11 MPa, and 7.22 MPa, respectively. The compressive modulus of these three groups had no statistically significant difference (p>0.05). In MTT assay, cell numbers of type II collagen-coated PCL scaffold were higher than PCL scaffold (77.4%) and gelatin-coated PCL scaffold (16.4%). In GAG assay, There were statistically significant difference (p<0.05) among three groups after four weeks and twelve weeks culture. The results of MTT and GAG assay showed that the type II collagen-coated PCL scaffold had better environment for cell proliferating and attaching. In the third part, a functional scaffold carrier with pressure sensing unit was designed and fabricated. The force-resistance curve of pressure sensing unit with and without PCL films were evaluated the property consistency of pressure sensing unit before and after imbedding into PCL films. it demonstrated that tissue engineering technique and sensing technique could be integrated as a functional sensing scaffold for continuously monitoring pressure on repair scaffold of cartilage