Chemical and Biological Investigations of the Roots of Euphorbia formosana Hayata

• Main Authors: Ting Ging Guo, 郭亭君
• Other Authors: Y. L. Leu
• Format: Others
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/45886007439134891129

碩士 === 長庚大學 === 中醫學系天然藥物 === 99 === Euphorbia formosana is one species of Euphorbiaceae. It is the native endemic plant in Taiwan. It is an perennial herbaceous plant and mainly distributed in low elevation sand. Forty compounds were isolated from the dried roots of E. formosana (15.00 kg). Thirty-five compounds were determined by spectral analysis and compared with authentic literatures. They are included ellagic acid (1), 3,3’-di-O-methylellagic acid (2), 3,3’,4,4’-tetra-O-methylellagic acid (3), 3,3’-di-O-methylellagic acid 4’-O-β-xylopyranoside (4), 3,3’-di-O-methylellagic acid 4’-O-β- glucoside (5), 3,3’-di-O-methylellagic acid 4’-O-β-arabinopyranoside (6), 3’-O-methyl-3,4-methylenedioxy ellagic acid (7), gallic acid (8), methyl gallate (9), methyl brevifolincarboxylate (10), brevifolin (11), phyllanthusiin E (12), dehydrochebulic acid trimethyl ester (13), octacosyl ferulate (14), β-sitosterol (15), β-sitosteryl-3-O-glucoside (16), β-sitostenone (17), ergosterol peroxide (18), aurantiamide acetate (19), 5-hydroxymethylfurfural (20), scopoletin (21), euoniside (22), 6-methoxy-7,8-methylenedioxycoumarin (23), helioscopinolide E (24), isopimara-7,15-dien-3-one (25), epi-manool (26), larixol (27), euphol (28), glutinone (29), cycloart-23- ene-3,25-diol (30), tirucalla-8,25-diene-3,24-diol (31), quercetin-3-O-α-L-rhamnoside (32), kaempferol-3-O-α-L-rhamnoside (33), 4-methyl-5,6-dihydropyran-2-one (34) and 1,3,4,6-tetra-O-galloyl-β-D-glucopyranose (35). The major compounds of E. formosana were polyphenols and terpenoids. The isolated compounds were evaluated their anti-inflammatory bioactivities. The results of anti-inflammatory activity showed that methyl brevifolincarboxylate (10), brevifolin (11) and larixol (27) have significant inhibitory activities on the release of superoxide anion in fMLP/CB-activated human neutrophils with IC50 values 0.68 ± 0.18, 1.39 ± 0.12 and 3.81 ± 0.43 μM, respectively.