Development of molecular serotyping, detection methods and analysis of antibiotic resistance of Salmonella spp. in environmental water

• Main Authors: Hsiao, Hsiangyu, 蕭翔宇
• Other Authors: Bing-Mu Hsu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/91370292514198624448

碩士 === 國立中正大學 === 應用地球物理研究所 === 99 === Salmonella spp. are widely distributed in the environment, which are found in livestock, wild animals and water. It can cause serious diseases in both human beings and animals, and infections with non-typhoidal Salmonella are a significant cause of illness and death worldwide.Furthermore, resistance to various classes of antimicrobial agents has been encountered in many bacteria of medical relevance. Particularly, attention has been paid to zoonotic bacteria such as Salmonella. In our study, we combined traditional culture method and molecular method to detect Salmonella in environmental water and to analyze its antimicrobial resistance. The aim of this study is to detect the occurrence of Salmonella from environmental water of Puzih stream and Kaupin River in Taiwan by polymerase chain reaction (PCR), then identify the serovar of Salmonella by PFGE and Multiplex PCR. At last, we use PCR and Disk Difussion Method to evaluate the antibiotic resistance of Salmonella. The occurrence frequency of Salmonella from Puzih stream was 21% (21/100) and Kaupin River was 40.35 % (23/57). The 44 positive water samples by culture method were further identified as S. Typhimurium (1/44), S. Bareilly (13/44), S. Isangi (3/44), S. Choleraesuis (2/44), S. Potsdam (1/44), S. Albany (10/44), S. Derby (4/44), S. Kedougou(3/44), S. Potsdam(1/44), S. Mbandaka(1/44), S. Oranienburg(1/44), S. Weltevreden(1/44), S. Agona(1/44) 以及 S. Newport (3/44)by biochemical testing, serological identification and PFGE. On the other hand, antibiotic resistance bacteria do exist in environmental water, and Salmonella spp. in environmental water also have the same trend of antibiotic resistance with clinical samples, including multidrug resistance Salmonella. Studies are needed to determine the sources of different multidrug-resistant Salmonella serotypes. Continued surveillance is needed to monitor changes in resistance trends and to detect further emergence of resistant Salmonella serotypes in Taiwan.