Differentiation of induced pluripotent stem cells in heparin and nerve growth factor-grafted polycaprolactone-poly-(3-hydroxybutyrate)

• Main Authors: Huang, Minjung, 黃敏榮
• Other Authors: Kuo, Yungchih
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/08005854426965861899

碩士 === 國立中正大學 === 化學工程研究所 === 99 === Induced pluripotent stem cells (iPSCs) have been considered as the critical issue in regeneration medicine. This study investigates the physicochemical properties of heparin and nerve growth factor (NGF)-grafted polycaprolactone (PCL)/poly(3-hydrobutyrate) (PHB) scaffolds and the NGF-guided differentiation of iPSCs in the scaffolds. The PCL/PHB scaffolds were fabricated by solvent merging/particulate leaching method. The PCL/PHB scaffolds with the mass ratio of 4:1 have a relatively high porosity and cell adhesion. In addition, an increase in the weight percentage of heparin enhanced the cell viability. The flow cytometry analysis of heparin and NGF-grafted PCL/PHB scaffolds demonstrated that when NGF was introduced, an increased weight percentage of PCL yielded more identified neurons. In addition, the immunocytochemical staining revealed that PCL/PHB scaffolds grafted with heparin and NGF could induce the differentiation of iPSCs into neurons via the expressed β-III tubulin. A proper control over the physical and biomedical property of the scaffolds and the NGF-guided differentiation of iPSCs can be promising for nerve regeneration.