Investigation of the Biochemical Mechanism of Cholesteatoma by Proteomic Analysis

• Main Authors: Kuo-Fong Hung, 洪國峰
• Other Authors: Yu-Jen Wu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/82423612973821013143

碩士 === 美和科技大學 === 健康與生技產業研究所 === 99 === Cholesteatoma is a commonly occurred disease in otolaryngology. It is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. A perforation of the eardrum caused by chronic infection or direct trauma could lead to cholesteatoma, which could cause destruction of three auditory ossicles located in the middle ear and result in hearing deterioration, deafness, physical imbalance, and vertigo. In this study, the expression changes of overall proteins between the tissues of cholesteatoma and those of retroauricular skin were detected. The roles of the differential proteins in the biomechanism of proliferation and differentiation of keratinocytes in middle ear cholesteatoma were investigated. Twelve patients participated in this study in collaboration with the Affiliated Hospital at the Kaohsiung Medical University. The cholesteatoma specimens were resected during surgical operations. The retroauricular skin samples of the patients were also resected during surgery as the controls. Comparative proteomic analysis was used to detect the differential proteins by comparing the 2-DE maps of the tissues of cholesteatoma with those of the retroauricular skin. A total of 88 differential proteins, including 73 up-regulated and 15 down-regulated proteins, have been identified by LC/MSMS analysis. The 2-DE data were further confirmed by reverse transcription-polymerase chain reaction, western blot analysis, and immunohistochemistry stain method. The differential proteins are related with protein folding, proliferation, inhibition of apoptosis, calcium binding proteins, protease inhibitor, cytoskeleton proteins, antioxidant proteins, and metastasis proteins. The functions of these proteins might be involved in biochemical pathways of the proliferation and differentiation of keratinocytes in cholesteatoma.