Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River

碩士 === 元智大學 === 生物科技與工程研究所 === 98 === The aim of this study was isolation and characterization of heavy metal resistant microorganisms from sediments of Er-Jen River in south Taiwan, a river that is well known to be polluted by various contaminants. We have isolated several microorganisms that are...

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Main Authors: Bo-Chou Lin, 林柏州
Other Authors: Chih-Ching Chien
Format: Others
Language:zh-TW
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/65499000026475357794
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spelling ndltd-TW-098YZU051110072015-10-13T18:20:57Z http://ndltd.ncl.edu.tw/handle/65499000026475357794 Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River 二仁溪沉積分離之假單孢菌形成生物膜與抗重金屬能力之探討 Bo-Chou Lin 林柏州 碩士 元智大學 生物科技與工程研究所 98 The aim of this study was isolation and characterization of heavy metal resistant microorganisms from sediments of Er-Jen River in south Taiwan, a river that is well known to be polluted by various contaminants. We have isolated several microorganisms that are highly resistant to heavy metals including cadmium by enrichment culture. One of the isolates was identified as a Pseudomonas sp. according to its 16S rRNA gene sequence analysis and was designed as strain EJ01. Pseudomonas sp. EJ01 was used for further characterization in this study. The minimum inhibitory concentrations of cadmium for strains EJ01 was 7 mM when cells grown in LB medium containing cadmium. At stationary phase, cells of Strain EJ01 aggregated and formed biofilm when concentrations of Cd was high (2 mM and above). This phenomenon might be related to the bacterium’s ability to produce extracellular polysaccharides (EPS). Therefore, EPS produced by strain EJ01 was extracted from stationary phase grown cells to investigate its relationship to the bacterium’s resistance to Cd and the removal of cadmium. Cadmium concentration was reduced 75% in the culture medium when Pseudomonas sp. EJ01 grown in the medium containing 2 mM. The extracted EPS also showed significant ability for adsorption of cadmium in the solution. Transposon mutagenesis was also used to study genes involved in biofilm formation in strain EJ01 and their possible roles in heavy metal resistance. A mutant (EJ01m-3055) was selected as reduced biofilm formation mutant. The mutant also showed decreased ability for cadmium resistance and suggested that biofilm formation and cadmium resistance in Pseudomonas sp. EJ01 is realted. Chih-Ching Chien 簡志青 2010 學位論文 ; thesis 39 zh-TW
collection NDLTD
language zh-TW
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description 碩士 === 元智大學 === 生物科技與工程研究所 === 98 === The aim of this study was isolation and characterization of heavy metal resistant microorganisms from sediments of Er-Jen River in south Taiwan, a river that is well known to be polluted by various contaminants. We have isolated several microorganisms that are highly resistant to heavy metals including cadmium by enrichment culture. One of the isolates was identified as a Pseudomonas sp. according to its 16S rRNA gene sequence analysis and was designed as strain EJ01. Pseudomonas sp. EJ01 was used for further characterization in this study. The minimum inhibitory concentrations of cadmium for strains EJ01 was 7 mM when cells grown in LB medium containing cadmium. At stationary phase, cells of Strain EJ01 aggregated and formed biofilm when concentrations of Cd was high (2 mM and above). This phenomenon might be related to the bacterium’s ability to produce extracellular polysaccharides (EPS). Therefore, EPS produced by strain EJ01 was extracted from stationary phase grown cells to investigate its relationship to the bacterium’s resistance to Cd and the removal of cadmium. Cadmium concentration was reduced 75% in the culture medium when Pseudomonas sp. EJ01 grown in the medium containing 2 mM. The extracted EPS also showed significant ability for adsorption of cadmium in the solution. Transposon mutagenesis was also used to study genes involved in biofilm formation in strain EJ01 and their possible roles in heavy metal resistance. A mutant (EJ01m-3055) was selected as reduced biofilm formation mutant. The mutant also showed decreased ability for cadmium resistance and suggested that biofilm formation and cadmium resistance in Pseudomonas sp. EJ01 is realted.
author2 Chih-Ching Chien
author_facet Chih-Ching Chien
Bo-Chou Lin
林柏州
author Bo-Chou Lin
林柏州
spellingShingle Bo-Chou Lin
林柏州
Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
author_sort Bo-Chou Lin
title Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
title_short Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
title_full Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
title_fullStr Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
title_full_unstemmed Heavy metal resistance and biofilm formation of Pseudomonas sp. isolated from sediment of Er-Jen River
title_sort heavy metal resistance and biofilm formation of pseudomonas sp. isolated from sediment of er-jen river
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/65499000026475357794
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