| Summary: | 碩士 === 元智大學 === 生物科技與工程研究所 === 98 === Tyrosinase is the main enzyme in the formation of melanin, currently, most researches use tyrosine inhibitors to inhibit the function of this enzyme so as to reduce the production of melanin to achieve whitening effect. Therefore, the whitening agents of skin care products available in the market mostly based on the mechanism of the inhibition of Tyrosinase activity in the formation process of melanin.
Many effective ingredients in Ganoderma lucidum and other mushrooms species such as Antrodia cinnamomea, Agaricus blazei Murrill, Hericium erinaceus , Cordyceps militaris have been used in health foods for many years, and many studies have already confirmed that through a appropriate separation, the effective ingredient of Ganoderma Lucidum,Antrodia cinnamomea, Agaricus blazei Murrill, Hericium erinaceum , Cordyceps militaris can be purified. Natural products or herbal extracts adding to whitening maintenance products for skin care products has been the demands of the mainstream in recent years, many well-known cosmetic companies are also committed to the development and application of whitening natural materials. This research aims to study Ganoderma lucidum and other edible mushrooms using culture mycelium of biotechnology fermentation, through the use of 75% ethanol, 50% ethanol and water, to conduct the extraction and test the tyrosinase inhibition activity of the extracts as well as screen mushrooms with tyrosinase inhibition effect. Preliminary results showed that the aglycon of 75% ethanol extracts , 50% ethanol extratcts, and 5% H2O extracts of Ganoderma lucidum at the concentration of 1000 ppm were up to 78.2%, 74.4% and 79.0%, respectively; And the aglycon of 75% ethanol extracts , 50% ethanol extratcts, and 5% H2O extracts of Ganoderma lucidum at the concentration of 100ppm were up to 40.3%, 26.7% and 36.7%, respectively; Compared to the extracts of other edible mushrooms of antrodia cinnamomea, Agaricus blazei Murrill, Hericium erinaceum , Cordyceps militaris, Ganoderma lucidum extracts were with higher activity in inhibiting the function of tyrosinase, and with no cell toxicity to HS68 human fibroblast cells. Thus, the extracts of Ganoderma lucidum may be a natural raw material with whitening effect.
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