Analysis of Anti-Oxidant ability supplements Antrodia Camphorate extracts by Capillary electrophoresis

• Main Authors: Tsung-Hsuan Lin, 林宗玄
• Other Authors: Hai-Lung Wang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/wgy74n

碩士 === 元培科技大學 === 生物技術研究所 === 98 === This study focuses on the rats with hyperlipidemia and their body’s physiological changes after exercise and intake of Antrodia Camphorate extract supplement. The 80 male Sprague Dawley rats are the experiment subjects; they were randomly assigned in the low, medium, and high-dose groups. A dose of 0.1 g / kg / day was administered for the low-dose group, 0.3 g /kg /day for the medium-dose group, and 1g / kg / day for the high-dose group. After administering the Antrodia Camphorate extract for 4 consecutive weeks, the rats were then sub-divided into the exhaustive exercise group and non-exhaustive exercise group that each consists of 10 rats. The rats of the exhaustive exercise group were first placed on a rodent treadmill of 10% slope where they warmed up for 10 minutes at 15 meters/minute, until the rats run up to failure. Afterwards, the rats were sacrificed to collect blood samples and remove the brain for analysis. The two factors of the Antrodia Camphorate extract supplement and exhaust exercise were then compared to determine the biochemical analysis values in the bloodstream of the rats, the blood lipid status, and oxidative stress changes. The experimental results have indicated that after intake of the Antrodia Camphorate extract supplement, the four biochemical analysis values of GPT, TG, LDG, and CPK found in the body have reached significant differences (p <0.05), but the results for the lipid peroxide MDA in the brain and the capillary electrophoresis analysis results for GSH and GSSG have not shown remarkable changes.