A disposable glucose biosensor based on Fe3O4-SiO2 nanocomposite modified electrode

• Main Authors: Siou-Rong Chen, 陳秀蓉
• Other Authors: Wen-Chang Chen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/85846603185916030903

碩士 === 雲林科技大學 === 化學工程與材料工程研究所 === 98 === Magnetic Fe3O4-SiO2 nanocomposite particles were prepared by the method of chemical co-precipitation, followed by the surface modification with –NH2 group by using APTES, and then were used to prepare a disposable and cost-effective glucose SPCEs biosensor, using ferricyanide as the mediator. The modified processes of the SPCEs and related electrochemical characteristics were analyzed by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. Results show that the electroactive area and kinetic constant of the Fe3O4-SiO2-APTES modified electrode were increased to 43.3-fold and 17.1-fold, respectively, due to its high degree of hydrophi- licity and biocompatibility. Under the applied potential of 0.4 V and 0.8 U GOD for 18 mg Fe3O4-SiO2-APTES/ml modified electrode, a high biosensor sensitivity of 0.45 μA/mM could be obtained with a linearity up to 33.3 mM.