The Study of Mesenchymal Stem Cells from Human Umbilical Cord of Wharton’s Jelly Improve the Rat Animal Model of Myocardial Infarction

• Main Authors: Shin-I Liu, 劉欣宜
• Other Authors: Hwai-Shi Wang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/20079646778604360060

碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 98 === Transplantation of human umbilical cord mesenchymal stem cells from Wharton’s jelly to the myocardium of myocardial infarct rat, which induced by left anterior descending artery ligation. Observation of the improvement of the left ventricular function and fibrosis following the human umbilical cord mesenchymal stem cell from Wharton’s jelly injection. In our experiments, 1×106 undifferentiated , TGF-β2 induced human umbilical cord mesenchymal stem cells from Wharton’s jelly and normal saline inject into the myocardium of myocardial infarct rat, and observation by echocardiogram, Masson’s Trichrome staining,immunohistochemistry, also, examine the ability of C-troponin-I, CK-MB in the serum to assess morphologically and functionally the undifferentiated and TGF- β 2 induced human umbilical cord mesenchymal stem cells from Wharton’s jelly repair the myocardial infarct rat. We find out that undifferentiated and TGF-β2 induced human mesenchymal stem cells from Wharton’s jelly improve left ventricular systolic function by lowering down the activity of cardiac enzyme and reducing the area of fibrosis of cardiomyocytes. Also, The retention of transplanted human mesenchymal stem cells from Wharton’s jelly start to express the cardiac marker—C troponin I .