Raman Probe for different AT-rich duplex DNA structures

• Main Authors: Meng-Ju Tsai, 蔡孟汝
• Other Authors: Ta-Chau Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/00434239984224102237

碩士 === 國立陽明大學 === 生醫光電工程研究所 === 98 === Take advantage of Raman spectroscopy technique to identify vibration mode of molecular，and BMVPA-4 having bare fluorescence is a Raman probe. From Hung-Chin Fang’s master thesis (department of chemistry,ntu,2009)，BMVPA-4 interacting with different structure DNA is diverse in Raman spectrum，especially in extraordinary duplex DNA. In my study，I study that different sequences in duplex DNA interact with BMVPA-4，and I hope that BMVPA-4 is a DNA probe for special sequences. In experiment，when BMVA-4 interacts with AT-rich duplex DNA，it shows red shift in vibration mode of 1543 cm-1 as DNA concentration increasing. BMVPA-4 binds with different AT-rich duplex DNA，and red shift in 1543 cm-1 are different from each others. Red shift in 1543 cm-1 and deconvolution in 1543 cm-1 can identify different compose of continuous A-T sequences，and than BMVPA-4 is minor groove binding with DNA. In conclusion，BMVPA-4 is a potential Raman probe of AT-rich duplex DNA. It will be a drug to be used for curing AT-rich cancer or diseases in future.