Inhibitory effect of eugenol on osteoclast differentiation and bone resorption

• Main Authors: Hisu-Min Chao, 趙琇民
• Other Authors: Ying-Chieh Tsai
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/18331476710149474836

碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 98 === Several major bone diseases are directly attributable to bone loss, including osteoporosis, bone metastasis, and rheumatoid arthritis. Osteoporosis reflected the enhancement of osteoclast activity. Therefore, blocking the differentiation and maturation of osteoclast is a possible strategy to address this problem. In this study, RAW264.7 macrophage cell line was used as cell model to find anti-osteoclastogenesis effect of essential oil. We found clove oils showed the inhibition effect of osteoclast differentiation, and identified the effective component is eugenol. The aim of this study was to investigate the inhibition effect of eugenol on osteolcastogenesis and the molecular mechanism. The results showed eugenol had the ability to suppress RANKL inducing osteoclast differentiation in a dose dependent manner, and the half maximal inhibitory concentration (IC50) of eugenol is 54.7 μM. The mRNA and protein expression of c-Fos and NFATcl were decreased under treatment of eugenol. The gene expression of OSCAR, CTR, DC-STAMP, TRAP, and MMP-9 induced by RANKL was also suppressed by eugenol. In addition, eugenol inhibited phospho-p38, phospho-ERK and NF-κB expression, and decreased the degradation of IκB. Finally, pit formation assay demonstrated eugenol can block bone resorption. In conclusion, we suggested eugenol shows potential as a treatment for osteoporosis.