Wheat Bran as the Substrate for Cellulase Production by Trichoderma reesei RUT C30 Under Solid-State Fermentation

• Main Authors: Hsin-Ting Kuo, 郭信廷
• Other Authors: Kow-Jen Duan
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/92655929946726670044

碩士 === 大同大學 === 生物工程學系(所) === 98 === Cellulase from Trichoderma reesei RUT C30 was produced by solid culture using wheat bran in the present study. The solid culture was performed in a 250 ml Erlenmeyer flasks. The maximum enzyme activity of 9.9 FPU/g-DW was achieved by the following optimum conditions: inoculum size, 10% (v/w); initial substrate moisture content, 40% (w/w); initial substrate pH, 5.0; temperature, 30℃; incubation time, 3 days. The optimum thickness of substrate of the solid culture was in the range of 1~2.5 cm by a tray fermenter. The optimal cellulase activity of the crude enzyme was at pH 4.8, and 50~60 C. Cellulase activity (filter paper unit, FPU) could increase up to 1.65 fold by addition of ??glucosidase to the crude extract from the solid culture. Addition of ??glucosidase could eliminate the inhibition of cellobiose to cellulase. Sugar hydrolyzed from rice bran or sugarcane bagasse by the crude extract of cellulase plus -glucosidase was similar to those from the AccelleraseTM 1000, the commercial cellulase of Genencor.