Production of high-content isomaltooligosaccharides by coimmobilized transglucosidase and yeast cell

• Main Authors: Shiau-Chi Chen, 陳筱祺
• Other Authors: Dey-Chyi Sheu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/64804219730047423136

碩士 === 大同大學 === 生物工程學系(所) === 98 === Isomaltooligosaccharides（IMO）produced through the catalytic reaction of transglucosidase on maltose contain glucose and several oligosaccharides which have at least one α-D-(1,6) glucosidic linkage. IMO is a functional food ingredient which has great potential to improve the quality of many foods. IMO is a prebiotic, because it is non-digestible and can stimulate the proliferation of bifidobacteria in human intestine. High content IMO produced by removing byproduct glucose from IMO is receiving increasing interest due to its low calories, no cariogenicity and safety for diabetics. This work fell into two parts. In part I the optimal concentration of crosslinker EDC for the immobilization of transglucosidase on alginate was investigated. In part II the temperature tolerant yeast was immobilized using the enzyme-immobilized alginate, forming beads of calcium alginate. In this dual-biocatalyst system, high content IMO was obtained from maltose due to the byproduct glucose being removed. The results showed that the optimum conditions were 45ºC and pH 5.5. Optimal stability and reusability of the immobilized transglucosidase were obtained when 0.5% EDC was used as the crosslinker. In addition, more than 80% on a dry weight basis of IMO was obtained, the immobilized dual biocatalyst worked for the production of high-content IMO from maltose.