Investigation of the Problems Associated with Capillary DNA Sequencing

• Main Authors: Sung-En Chen, 陳頌恩
• Other Authors: Dey-Chyi Sheu
• Format: Others
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/25427034270205586955

碩士 === 大同大學 === 生物工程學系(所) === 98 === All creatures on earth are constructed with cells. Cells contain genetic materials which are made of DNA. All living things are characterized with the proteins expressed by codes on their DNA. DNA is a polymer of nucleotides. Each nucleotide is composed of pentose, phosphate, purine or pyrimidine. The genetic message is governed by the sequence of purine and pyrimidine. Sanger method which is specified by selective termination of DNA-polymerase, is a primary process to read the codes on DNA. The modern DNA-sequencing technique, an important tool in molecular biology, is a combination of Sanger method and capillary electrophoresis. Success in DNA-sequencing depends on the properties of sample and primer, and the skill of experimental operator. The factors affecting the results of DNA-sequencing are investigated in this work.