The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles

碩士 === 臺北醫學大學 === 醫學科學研究所 === 98 === Abstract Given the intensive nano science and technological development, the health effect of nanosized products to human beings had been concerned. Nono-materials’ toxicological assessment on HepG2 cells were conducted after co-incubated with nano-sized ZnO and...

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Main Authors: Yi-Ling Liu, 劉怡伶
Other Authors: 麥富德
Format: Others
Language:en_US
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/03432483278749296399
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spelling ndltd-TW-098TMC055500672016-04-22T04:23:31Z http://ndltd.ncl.edu.tw/handle/03432483278749296399 The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles 奈米粒子ZnO與TiO2之危害與保護 Yi-Ling Liu 劉怡伶 碩士 臺北醫學大學 醫學科學研究所 98 Abstract Given the intensive nano science and technological development, the health effect of nanosized products to human beings had been concerned. Nono-materials’ toxicological assessment on HepG2 cells were conducted after co-incubated with nano-sized ZnO and TiO2. Nanosized zinc oxide was coated with a natural product, dextran, to minimize its aggregate formation and appeared its original form while exposure to human beings. The ZnO particle-medium was cytotoxic but the TiO2 one was not. Associating with two high technical imaging systems, the laser confocal microscopy analysis pointed out the locations and condition of organelles in HepG2 cells after they treated with nanoparticles and then fluorescent dye; TOF-SIMS imaging offers a modality for simultaneously the spatial distribution of nanosparticles in cells. Combination of these two high technical images, it showed the effects to each organelle and the oxidative stress by nanoparticles and their distribution in HepG2 cells. To summarize all the primary data we had, we also expect the next steps that when cells were exposed to ZnO nanoparticles, a temporal pattern of apoptotic events was observed following the elevation of O2-, in which cytochrome c release and mitochondrial depolarization preceded caspase-3 activation and DNA fragmentation. Moreover, we expect to prove the cell proliferation phenomena by immunoblot assay after cells exposed to TiO2 nanoparticles. The primary data showed the morphology changes and also cells’ response, but it most still need to be identified by other techniques. After this, we will focus on tracing the really pathway or impacts from HepG2s to get more biochemical mechanism at this condition. This study highlighted the possibility for caution during the produce and purchase of nano-materials to prevent human health impacts. 麥富德 2010 學位論文 ; thesis 58 en_US
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description 碩士 === 臺北醫學大學 === 醫學科學研究所 === 98 === Abstract Given the intensive nano science and technological development, the health effect of nanosized products to human beings had been concerned. Nono-materials’ toxicological assessment on HepG2 cells were conducted after co-incubated with nano-sized ZnO and TiO2. Nanosized zinc oxide was coated with a natural product, dextran, to minimize its aggregate formation and appeared its original form while exposure to human beings. The ZnO particle-medium was cytotoxic but the TiO2 one was not. Associating with two high technical imaging systems, the laser confocal microscopy analysis pointed out the locations and condition of organelles in HepG2 cells after they treated with nanoparticles and then fluorescent dye; TOF-SIMS imaging offers a modality for simultaneously the spatial distribution of nanosparticles in cells. Combination of these two high technical images, it showed the effects to each organelle and the oxidative stress by nanoparticles and their distribution in HepG2 cells. To summarize all the primary data we had, we also expect the next steps that when cells were exposed to ZnO nanoparticles, a temporal pattern of apoptotic events was observed following the elevation of O2-, in which cytochrome c release and mitochondrial depolarization preceded caspase-3 activation and DNA fragmentation. Moreover, we expect to prove the cell proliferation phenomena by immunoblot assay after cells exposed to TiO2 nanoparticles. The primary data showed the morphology changes and also cells’ response, but it most still need to be identified by other techniques. After this, we will focus on tracing the really pathway or impacts from HepG2s to get more biochemical mechanism at this condition. This study highlighted the possibility for caution during the produce and purchase of nano-materials to prevent human health impacts.
author2 麥富德
author_facet 麥富德
Yi-Ling Liu
劉怡伶
author Yi-Ling Liu
劉怡伶
spellingShingle Yi-Ling Liu
劉怡伶
The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
author_sort Yi-Ling Liu
title The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
title_short The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
title_full The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
title_fullStr The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
title_full_unstemmed The Hazard and Protection Effects of ZnO Nanoparticles and TiO2 Nanoparticles
title_sort hazard and protection effects of zno nanoparticles and tio2 nanoparticles
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/03432483278749296399
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