| Summary: | 碩士 === 臺北醫學大學 === 醫學科學研究所 === 98 === Abstract
Given the intensive nano science and technological development, the health effect of nanosized products to human beings had been concerned. Nono-materials’ toxicological assessment on HepG2 cells were conducted after co-incubated with nano-sized ZnO and TiO2. Nanosized zinc oxide was coated with a natural product, dextran, to minimize its aggregate formation and appeared its original form while exposure to human beings. The ZnO particle-medium was cytotoxic but the TiO2 one was not. Associating with two high technical imaging systems, the laser confocal microscopy analysis pointed out the locations and condition of organelles in HepG2 cells after they treated with nanoparticles and then fluorescent dye; TOF-SIMS imaging offers a modality for simultaneously the spatial distribution of nanosparticles in cells. Combination of these two high technical images, it showed the effects to each organelle and the oxidative stress by nanoparticles and their distribution in HepG2 cells. To summarize all the primary data we had, we also expect the next steps that when cells were exposed to ZnO nanoparticles, a temporal pattern of apoptotic events was observed following the elevation of O2-, in which cytochrome c release and mitochondrial depolarization preceded caspase-3 activation and DNA fragmentation. Moreover, we expect to prove the cell proliferation phenomena by immunoblot assay after cells exposed to TiO2 nanoparticles. The primary data showed the morphology changes and also cells’ response, but it most still need to be identified by other techniques. After this, we will focus on tracing the really pathway or impacts from HepG2s to get more biochemical mechanism at this condition. This study highlighted the possibility for caution during the produce and purchase of nano-materials to prevent human health impacts.
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