Study on the antioxidant activity of coumarin derivatives

• Main Authors: Yu-Shiang Chou, 周裕翔
• Other Authors: Duen-Suey Chou
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/73140093187127013036

碩士 === 臺北醫學大學 === 醫學科學研究所 === 98 === In our previous study, we demonstrated that baicalein induced superoxide and hydroxyl radical formation in B16F10 mouse melanoma cell line. We also found the reactive oxygen species (ROS) production is related to the arachidonic acid (AA) metabolism way. Simultaneously baicalein caused reduction of cellular viability and cell apoptosis. In this study, we use electron spin resonance (ESR) to analyze different systems, investigating the antioxidant activity of coumarin derivatives. System I using DPPH and found that coumarin derivatives all have the antioxidant activity. System II is xanthine oxidase / hypoxanthine system, directly investigating the superoxide intensity, and we found that coumarin derivatives also all have the antioxidant activity. System III directly analyze baicalein induced hydroxyl radical formation in B16F10 mouse melanoma, we found that coumarin derivatives Mei-174、Mei-215、Mei-228 and Mei-234 have the antioxidant activity. We also use coumarin derivatives to observe whether they can reduce the reduction of cellular viability effect to baicalein in B16F10 cell by MTT assay, the data show that coumarin derivatives have no obvious effect on cellular 4 viability. The antioxidant activity of coumarin derivatives in different systems can refer to the future study of their mechanism.