| Summary: | 碩士 === 臺北醫學大學 === 臨床醫學研究所 === 98 === Background: Diabetic nephropathy is one major cause of renal failure, and poor glycemic control will increase cardiovascular incidence. Chronic high glucose condition induced oxidative stress, increased reactive oxygen species (ROS) amount, and involves kidney mesangial cell growth arrest and apoptosis pathway. We investigated the therapeutic effect of resveratrol, a polyphenolic compound, with multi-function nature phytoalexin may attenuate high glucose induced cell arrest and apoptosis changes in mouse mesangial cell via anti-oxidant and anti-inflammation proprieties. Material and methods: Mouse mesangial cell were cultured in 6.25mM and 35mM glucose, and add resveratrol in high glucose treated group. Observe cell morphology and viability by microscopy and MTT test. Intracellular ROS generation was assayed by H2-DCFDA. Cell apoptosis amount was determined by flow cytometry via Annexin-V and propidium iodine staining. TGF and HO-1 gene expression was measured by reveres transcriptase- polymerase chain reaction. And caspase 3 activity was quantified by ELISA. Result: High glucose depressed cell growth, increases cellular ROS (~70 %; p < 0.05), and enhanced cell apoptosis (1.5~1.8 fold increase; p < 0.05); these reaction could be attenuated by physiological resveratrol concentration 1-2.5μM. And also noted decreased TGF-β and mediated HO-1 gene expression. Caspase 3 activity was also blocked (p < 0.05). Conclusion: Our findings indicate that resveratrol provides antioxidant effect against high glucose induced cell apoptosis, and may further regulate mesangial cell numbers.
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