Polymorphisms in Angiogenesis-Related Genes and Urothelial Carcinoma

• Main Authors: Chi-Tung Liu, 劉奇東
• Other Authors: Hung-Yi Chiou
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/07626334912525042163

碩士 === 臺北醫學大學 === 公共衛生學研究所 === 98 === Introduction： Urothelial carcinoma (UC) including bladder, renal pelvis and ureter cancer. Cancer staging and grading is the most important index for cancer prognosis, and angiogenesis is a necessary step in tumor growth and metastasis. This study was to discuss the relationship between five SNPs on the four angiogenesis-related genes：FIH-1, VHL, HIF-1, VEGF and the risk of UC morbidity, clinical pathological characteristics, recurrence, and survival rate. Methods： In this case - control study, we selected 850 cases of urothelial carcinoma and the age and sex matched 850 healthy controls. Data collation includes questionnaire and clinical pathological characteristics. Genotyping is determined by PCR-RFLP and Real-time PCR. Results： We found that Study subjects with cigarette smoking, alcohol consumption, occupational exposure, arsenic exposure, and high-salt diet have a significantly higher UC risk of 2.0, 1.6, 1.8, 1.9, and 1.5. Occupational exposure who has 2.2 times the risk of more serious clinical and pathological characteristics, arsenic exposure has 1.7 times increased risk of death. Smokers with VHL CC genotype have a higher UC risk of 2.4. FIH with CG/GG genotype had 1.3 times risk of developing to T2 and stage II or more serious. Smokers with VEGF-460 TT genotype had 1.6 times risk of developing to stage II or more serious. Combine the five SNPs risk genes found that the number of risk genes≧2 have a higher UC risk of 1.3 and 1.6 times risk of developing to T2 and stage II or more serious. The effect of SNPs around the smokers is more obvious. Conclusions： These results suggested a significant association between angiogenesis-related genes polymorphisms and UC morbidity and tumor stage, but not support a significant association between SNPs and tumor grade. The cigarette smoking confounds the association between polymorphisms and UC.