Longitudinal metabolite profile and effects of arsenite toxicity for zebrafish：studies by vitro NMR spectroscopy and 1H NMR metabonomics

• Main Authors: Chieh-ting Tsai, 蔡杰廷
• Other Authors: Chang-Shin Lee
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/61440973580001429356

碩士 === 淡江大學 === 生命科學研究所碩士班 === 98 === Arsenic is common compounds in nature, and caused many public health problems in Taiwan. Organisms take in arsenic lead to abnormal physiological function. Low concentrations promote cell proliferation. High concentrations inhibit the metabolism and cause multiple organ disease. Generally, inorganic arsenic is usually more toxic than organic arsenic. Organisms take in inorganic arsenic the detoxification pathway is through the methylation into organic arsenic. The zebrafish is an animal model in this thesis. Use solvent to extraction and freeze-dried the zebrafish product. Analyse metabolites include determination and quantification by NMR. Observe different arsenic conditions the metabolism research of zebrafish. Experiment was found the zebrafish is enhanced methylation metabolism by arsenic. TMAO can as indicator metabolite of health status in zebrafish. Confirmed the combination of zebrafish and NMR provide a viable way of metabolic study.