Effect of electrolytes on removal of protein from hyaluronic acid solution with using diafiltration

• Main Authors: Yan-Wen Zhong, 鍾燕雯
• Other Authors: 鄭東文
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/87490830673721529907

碩士 === 淡江大學 === 化學工程與材料工程學系碩士班 === 98 === This study investigated the effect of electrolytes on removal of protein(lysozyme,LY) from hyaluronic acid(HA) solution with using diafiltration in a dead-end stirred cell. The flux behavior, filtration resistance and HA/LY separation efficiency were discussed under various operating parameters such as electrolyte types, electrolyte concentrations, stirred rates, feed concentrations and diavolumes. Experimental results showed that the permeate flux decreased with the increase of ion strength of electrolyte which caused the HA molecules to form a more compact deposited layer on membrane surface. Meanwhile, the LY molecules did not aggregate because the surface charge of LY was covered by the electrode ion strength. The isolated LY molecules can pass through the membrane more smoothly. Therefore, the reduction of LY from the mixture solution of HA/LY increased with in creasing the ion strength, the permeate flux can be effectively enhanced by the addition of stir that disturbed the polarization layer of HA and reduced the filtration resistance. In diafiltrating the HA/LY solution, the step increase of electrolyte concentration in dialyate solution has significant.