| Summary: | 碩士 === 國立臺北科技大學 === 生物科技研究所 === 98 === This study explore the decolorization and degradation mechanism of textile dye, including reactive red dye HE3B (C.I. Reactive red 120) and direct green dye BB50 (C.I. Direct green B), through coculture of fungi and bacteria. After 24 hours cultivation, the decolorization efficiency of Aspergillus niger and Bacillus sp.was 50% and 30% respectively. However, the dye decolorization can reach 90% by using two strains coculture. Therefore, we designed Aspergillus niger and Bacillus sp. coculture model to enhance the performance of decolorization and degradation of dyes.
In order to find the optimal result of coculture, and explore the importance of different independent factor in dye biodegradation, we use the response surface methodology to achieve quicker and higher degree decolorization. Preliminary results showed that elements of the composite culture medium, such as malt extract, glucose, KH2PO4 concentration displayed a statistical significance in dye decolorization. Through the response surface method, reactive red dye HE3B had 92.68% decolorization and direct green dye BB50 had 97.66% decolorization in case of 0.1% malt extract, 0.7% KH2PO4 and 0.8% malt extract, 0.5% glucose, respectively, after 2 hours culture.
The chemical structure of decolorized residues had been analysised by HPLC. Based on the results, there are no any aniline compounds in the medium. Therefore, the aromatic structure had been destroyed in the decolorized procedure and did not produce any toxic components. Through immobilization of two strains on an insoluble substrate, the efficiency of decolorization can indicate higher efficiency and convenience. There exists a 3-fold better decolorization of immobilized mixed bacterial than the free state. So this strategy could be applied to wastewater treatment of textile plant for dye decolorization.
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