| Summary: | 碩士 === 東海大學 === 畜產與生物科技學系 === 98 === Conjugated linoleic acid (CLA) plays an important role in regulation of arachidonic acid metabolism. The way to raise the CLA content in the diet can reduce the allergen induced atopic asthma. CLA and lactic acid bacteria can modulate inflammation in bronchiolar hyperresponsiveness. The previous studies indicated that CLA-enriched milk can attenuate allergic airway disease in mice, but the mechanism is unclear. In this study, we administered yoghurt with or without various concentrations of CLA to determine their effects on OVA-induced airway hyperresponsiveness in BALB/c mice. Leukocytes harvest from bronchoalveolar lavage fluid (BALF), spleen, airway-draining and mesenteric lymph node were measurement. Cluster of differentiation markers including CD3, CD4, CD8, CD19, CD25, CD45, CD69, CD278, Tim-3 and PanNK, were used to distinguish the cells differentiation. The enhanced pause (Penh) was measured with whole body plethysmograpy to monitor the airway hyperresponsiveness. Specific antibodies including anti-OVA antibodies and cytokines in serum and BALF were measured with ELISA. The H&E and toluene blue stain of lung inflammation and mast cell infiltration were determined by microscopy. The results indicated that yoghurt and CLA enrichment restore the regulatory T cells (Treg) that increased the suppressor T cells in modulated immune response. Yoghurt and CLA could reduce the Th2 (CD278+, CD4+) and increase the Th1 (Tim-3+, CD4+) to modulate T helper cell differentiation. Another results indicated that CLA-enriched yoghurt significantly inhibited allergen-specific IgE production and reduced IL-6 in BALF. Mast cell infiltration was also reduced and decrease airway hyperresponsiveness in allergic BALB/c mice. In conclusion, CLA-enriched yoghurt is an idea healthy daily supplement for airway hypersensitivity via modulating Treg, Th1/Th2 differentiation and allergic IgE.
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