| Summary: | 碩士 === 亞洲大學 === 生物科技學系碩士班 === 98 === Hyaluronic acid (HA) is a natural polymer used in biomedical and cosmetic applications, and which has great water-holding capacity and high viscosity. HA also contributes significantly to various cellular processes such as adhesion, migration, and proliferation, as well to assist in the control of angiogenesis. Gold nanoparticles with the great stability present fascinating antioxidant activity and biocompatibility. HA and gold nanoparticles are the potential biomaterials to apply in medicine, drug delivery and cosmetics. In this study, gold-hyaluronic acid nanoparticles were prepared by chemical reduction method, and various particle sizes have been synthesized by the different concentration of the reducing agent. The absorption spectra of gold-hyaluronic acid nanoparticles measured by UV-visible spectrophotometer were observed near 520 nm. Their particle sizes and shapes were investigated by transmission electronmicroscope (TEM), which indicated the formation of uniform spherical particles with well dispersion (2-6nm). Therefore, the synthesis of size-controlled gold-hyaluronic acid nanoparticles could be achieved by chemical reduction methods, and the resultant of gold-hyaluronic acid nanoparticles is very stable and showed non-toxicity to NIH3T3 cell. The DPPH scavenging capacity is 52.79%, suggested as a candidate molecule for antioxidant. Pre-coating HA treat to tumor cell line (MCF7) showed anti-adhesion activity and inhibition rat is 43.49%. the results suggest gold-hyaluronic acid nanoparticles have potential in medicine applications, such as biomaterial and anticancer treatment.
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