| Summary: | 碩士 === 慈濟大學 === 微免暨分子醫學研究所 === 98 === Interferon-gamma-inducible protein-10 (IP-10, CXCL10) is an important chemokine and is thought to be chemotactic to activated T cells. In this study, we investigated the expression of IP-10 during murine Listeria monocytogenes (LM) infection. Using in situ immunofluorescent staining, unexpectedly, na��ve spleens from C57BL/6 mice were found to constitutively express high levels of IP-10. When the mice were infected with 1 x 104 CFU of LM, the expression levels of IP-10 decreased from day 4 to day 7 after infection and returned to the level as na��ve spleen 10 days post-infection. Interestingly, when giving the mice a higher dose of LM (5 x 104 CFU), the expression of IP-10 diminished rapidly with obvious decrease at 24 h post-infection. These results indicate that the inflammatory signals induced by LM infection are responsible for the down-regulation of IP-10 expression and that intensity of signals is correlated with kinetics of IP-10 down-regulation. We also delineated the expression of IP-10 to white pulp region of spleen. Using F4/80+ staining to outline red pulp, IP-10 staining and F4/80+ staining were found to be mutual excluded. On the contrary, both T cell and B cell regions were co-localized with IP-10 staining. To further solidify our result, protein levels and mRNA levels of IP-10 were currently under investigation with Western blotting and RT-PCR respectively.
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