Induction of Apoptosis by 8-shogaol via Reactive Oxygen Species Generation, Glutathione Depletion and Caspase Activation in Human Leukemia Cells

• Main Authors: YI-OWN CHEN, 陳宜萬
• Other Authors: PO-CHUEN SHIEH
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/81731770707768238387

碩士 === 大仁科技大學 === 製藥科技研究所 === 98 === Ginger, the rhizome of Zingiber officinale, is a traditional medicine with carminative effect, anti-nausea, anti-inflammatory, and anti-carcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that 8-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of the glutathione that committed to [8]-shogaol-induced apoptosis in HL-60 cells.