Research on proceeded Argyi leaf in Taiwan for wound healing

• Main Authors: Jung-Chieh Lee, 李榮傑
• Other Authors: I-Min Liu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/94863939851339966009

碩士 === 大仁科技大學 === 製藥科技研究所 === 98 === Argyi is the dried leaf of Artemisia plants (Compositae), which are distributed widely in Taiwan and Mainland. The acute toxicity of the extract of proceeded Argyi was evaluated, and indicating that mortality in mice was increased when the daily oral dosage was higher than 5 g. Moreover, the crude extract of proceeded Argyi was given orally once a day into Wistar rats for 28 days; it was observed that the repeatedly administration of water extract of Argyi at high oral dosage (600 mg/kg/day) can lead to liver damage and kidney inflammation. The degree in hepatotoxicity and nephrotoxicity induced by Argyi cannot relive by proceeding under high temperature. 　　The water extract of Argyi or Chi-Ai, posed similar inhibitory activity on tyrosinase, which is independent on proceeding temperature. Furthermore, remarkable cytotoxic activity of waster extract of Chi-Ai especially, against B16 melanoma cells was found; the activity is also independent on proceeding temperature. The potential of aqueous extract of Argyi in wound healing was further evaluated. The ointment with aqueous extract of Argyi showed significant anti-inflammatory activity in excision wound tissue. The rate in epidermal and granulation tissue formation on wound of normal Wistar rats was enhanced by ointment containing aqueous extract of Argyi. Moreover, the levels in transforming growth factor-β on wound of normal Wistar rats was increased by aqueous extract of Argyi, that leads to enhance the expression of collagen and fibronectin, ultimately resulting in wound healing. Similar wound healing effects induced by aqueous extract of Argyi on diabetic rats was obtained. However, aqueous extract of Argyi made no irritation on skin and eyes of rabbits. The results suggest that aqueous extract of Argyi has favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars. Results of the present study are helping in the understanding of biological activities and action mechanism on Argyi or Chi-Ai in Taiwan and Mainland. In addition, the information provided by our studies will be valuable to be the references for Argyi proceeding in pharmaceutical or cosmetic industry in the future.