Molecular Analysis of the Effects on the Testis and Osteoblast Cells by Brominated Flame Retardants.

• Main Authors: Chun-Chao Huang, 黃俊超
• Other Authors: Cying-Wen Ying
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/47520710650095166473

碩士 === 東吳大學 === 微生物學系 === 98 === Polybrominated diphenyl ethers (PBDEs) and tetrabromobisphenol A (TBBPA) are two commonly used brominated flame retardants. Because PBDEs have been banned by lots of countries, the role of TBBPA has become more important and gradually replaced PBDEs as the most widely used BFRs in recent years. Precious reports have indicated that BFRs could accumulate in animal tissues through different ways, including food chain, household dust, breast milk and placenta. Therefore, BFRs have bioaccumulation and biomagnifications theses two characteristics. This study is aimed to investigate the impacts of BFRs on Mus musculus leydig cell, sertoli cell and ICR mouse osteoblast primary cell, and also the gene expression profiles after feeding TBBPA to Wistar rat for 32 days. The results showed that TBBPA inhibits the growth of leydig cell and sertoli cell on high concentration (10-4 M、2×10-4 M). On the other hand, co-treatment with DHEA could interfere the normal function of DHEA on low concentration (10-8~10-5M). TBBPA also stimulated IL-1α and IL-6 gene expression, causing testicular excessive inflammation. Animal experiment found out that the weight of several organs showed no significant difference. In addition, microarray analysis has identified that 176 genes were regulated by TBBPA-64 genes are up-regulated and 112 genes are down-regulated. Then use Ingenuity Pathway Analysis (IPA) to cluster genes. These genes are known to be involved in the cellular development, cell growth and proliferation, cell death etc. In conclusion, it is not clear for the impacts of TBBPA accumulating in the body, but the impacts of BFR on endocrine function and testis development are worth further investigating.