| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 98 === Natural essential oils are extracted from different plant parts of aromatic crops, which has anti-bacterial, anti-infection effect, and the characteristics of plant essential oils are fat-soluble and small molecules that can easily penetrate the skin and mucous membranes. Essential oils have been widely used for bactericidal, virucidal, fungicidal, insecticidal, medicinal and cosmetic applications. Recently many studies are focusing on the antioxidant activities and major active biomoleculars in plant essential oils. However, no scientific satudy demonstrates the antioxidant and anti-inflammatory activities of essential oil from Vetiveria zizsnioidrs and Glechoma hederacea. Many researches indicate that free radicals are the major further in inflammatory conditions of several dieases, therefore the antioxidants may reduce and protect against inflammation. The purpose of this study is to investigate the antioxidant and anti-inflammatory activities of Vetiveria zizanioides and Glechoma hederacea essential oils.
The results of in vitro study had shown that both the tested essential oils possessed antioxidative characteristics including 2,2‘-azino-bis (3-ethylbenz-thiazoline-6-sulfonicacid) radical-scavenging effect and reducing power. Furthermore our results indicated that treatment of RAW 264.7 macrophages with Vetiveria zizanioides and Glechoma hederacea essential oils significantly decreased LPS-induced NO (nitric oxide) levels, superoxide anion production, and reduced thiobarbituric acid-reactive substances (TBARS) accumulation, increased the GSH level and regulated the antioxidant enzyme activities. The results showed that Vetiveria zizanioides and Glechoma hederacea essential oils may ameliorate oxidative stress in LPS-stimulated RAW 264.7 macrophages. Furthermore, both tested essential oils can prevents LPS-induced DNA fragmentation in RAW 264.7 macrophages, and reduce the LPS-induced RAW 264.7 macrophages mRNA expression including iNOS, COX-2, IL-1β, TNF-α and IFN-β mRNA expression. Additionally, both the tested essential oils can increase HO-1 mRNA expression in LPS-induced RAW 264.7 macrophages. The increasing HO-1 mRNA expression was reported that can protect cells from inflammation.
In conclusion, our study has established the information of antioxidant and anti-inflammatory potential of Vetiveria zizanioides and Glechoma hederacea essential oils and the molecular mechanism should be further explored.
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