| Summary: | 碩士 === 靜宜大學 === 化粧品科學系碩士班 === 98 === In the present work, vitamin B family, including B 1, B 2, B 3, B 5, B 6, B 7, B 9, and B 12 , for cosmetic applications were evaluated by antioxidation, whitening UV block determination and protecting tests for UV- induced damage of normal human skin fibroblasts Detroit 551 cells.Experiments include DPPH radical scavenging activity, chelating ferrous ions, Reducing power, ABTS+ radical scavenging activity, superoxide anion scavenging activity, Superoxide anion scavenging activity in B16F10 cells, inhibition of Dopachrome formation , melanin content in B16F10 cells, tyrosinase activity in B16F10 cells and UV absorption.
Vitamin B 1 at 0.05 mg / mL scavenging 84.9% of the ABTS + radicals, at 10 mg / mL scavenging 88.3% of the DPPH free radicals, in 30 mg / mL scavenging 77.0% of the superoxide anions, and at 50 μg / mL shows a significant (p <0.05) inhibition effect to reduce 34.3% of the superoxide anions in the B16F10 cells and to increase the cell viability of Detroit 551 cells damaged by UV-B or UV-A irradiation.
Vitamin B 3 at 10 mg / mL scavenging 76.7% of the superoxide anions, and inhibit 96.7% of the formation of Dopachrome the melanin intermediates, at 50 μg / mL can significantly (p <0.05) reduce 25.5 % of the amount of superoxide anions in the B16F10 cells and reduce 23.7% of the melanin content , and remarkably increase the cell viability of Detroit 551 cells damaged by UV-B or UV-A irradiation.
Vitamin B 6 at 0.05 mg / mL scavenging 92.7% of the ABTS + radicals, at 30 mg / mL scavenging 96.3% of the superoxide anions, and at 50 μg / mL shows a significant (p <0.05) inhibition effect to reduce 25.5% of the superoxide anions in the B16F10 cells and to increase the cell viability of Detroit 551 cells damaged by UV-B or UV-A irradiation.
Vitamin B 9 at 0.05 mg / mL scavenging 99.1% of the ABTS + radicals, at 1 mg / mL scavenging 62.9% of the superoxide anions, and at 50 μg / mL shows a significant (p <0.05) inhibition effect to reduce 17.0% of the superoxide anions in the B16F10 cells and to increase the cell viability of Detroit 551 cells damaged by UV-B or UV-A irradiation.
The results suggest that each of Vitamin B1, B 3, B6 or B9 can be used as a good antioxidant and can protect the cells from the injury by UV light , in addition, Vitamin B3 has a extra effect to inhibit the formation of melanin for whitening.
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