Inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells.
碩士 === 國立虎尾科技大學 === 生物科技研究所 === 98 === Mouse B16 melanoma cells and zebrafish were used as an in vitro and an in vivo system to evaluate the depigmenting activity of tested drugs. Among the screened crude extracts from selected Chinese herbs, a crude extract from Pemphis acidula was found to show st...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2010
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| Online Access: | http://ndltd.ncl.edu.tw/handle/2y9fk7 |
| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 98 === Mouse B16 melanoma cells and zebrafish were used as an in vitro and an in vivo system to evaluate the depigmenting activity of tested drugs. Among the screened crude extracts from selected Chinese herbs, a crude extract from Pemphis acidula was found to show strong depigmenting activity on melanogenesis of both B16 cells and zebrafish embryos. The inhibitory effects of the crude extract on melanogenesis were investigated in mouse B16 melanoma cells. The cytotoxicity IC50 value of the extract toward B16 cells was determined to be 211 μg/ml by a MTT method. In the other hand, the extract inhibited melanogenesis of B16 cells in a dose-dependent manner with an IC50 value of 33.3 μg/ml. Moreover, the inhibitory activity of the extract from Pemphis acidula also showed more potent than that of arbutin, which is a well-known commercial skin-whitening agent. The inhibitory mechanism of the extract on melanogenesis was to decrease the expression of tyrosinase by MITF down-regulation in the B16 cell.
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