Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein

• Main Authors: Po-Chin Chuang, 莊博今
• Other Authors: Yeung-Haw Ho
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/40598908448481294075

碩士 === 國立高雄大學 === 應用化學系碩士班 === 98 === The purpose of this study was to develop an online solid-phase extraction method that can increase the detection limit of capillary electrophoresis for analyzing protein. The method involved a SiO2 capillary column that inner surface fabricated with polyacrylic acid(PAA). Lysozyme in the sample was absorbed on the fabricated surface by ionic interactions between PAA and lysozyme. Adsorption and desorption of lysozyme was achieved by manipulating pH and ionic strength of sample solution. Several experimental conditions, conventional and microwave heating methods, size of PAA and SiO2 particle, were studied to maximize concentrated efficiency of lysozyme. SiO2 particle was used to derive the optimized conditions for fabrication. The optimized parameter was further applied on fabrication of capillary column.The amount of lysozyme extracted by PAA fabricated SiO2 particle (SiO2@PAA) was 7 times higher than that of corresponding bare SiO2 particle. Solution contained 100 ppm lysozyme was concentrated 15 times by SiO2@PAA. Nevertheless, only around 60% of adsorbed lysozyme was desorbed from SiO2@PAA. The amount of lysozyme that condensed by capillary column was double after the column fabricated with PAA. The result indicted that, after further improvement on concentrated efficiency, capillary column fabricated with PAA can be potential online solid-phase extraction tool for capillary electrophoresis.