| Summary: | 碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 98 === This study investigated the induction of human adipose tissue-derived stem
cells (hASCs) into chondrocytes, which made a possible source for cartilage
tissue engineering in clinical therapy. The hASCs were cultured on three
different biocompatible biomaterial groups including gelatin/collagen I: PCL
(GCI), gelatin/collagen II:PCL (GCII) and gelatin:PCL (GP). Considering the
high degradability of natural biomaterials, the addition of PCL for formulation
of scaffolds is necessary to resist absorption in vitro. In addition, we
characterized the attachment, growth and differentiation abilities of hASCs in
biomaterials by SEM, fluorescence microscopy and cell growth curve in vitro.
The immunofluorescence data showed that the hASCs were differentiated with
the increasing time on biocomposite membrane groups. For investigation of
cartilage differentiation, alcian blue staining and dimethylmethylene blue
(DMMB) assay were used for both quality and quantity evaluation of
glycosaminoglycans. The results showed that the groups of gelatin and GCII
contained more glycosaminoglycans than GCI. For further distinguishing
distinct collagen types produced by chondrocytes based on different
biocomposite membranes, sirius red staining was used and type I collagen
showed the absorbance at 540 nm and type II collagen at 350nm with
UV-visble spectrophotometer. We demonstrated that the chondrocytes release
mainly type I collagen on all biocomposite membranes. In detecting the long
term expression of calcium accumulation in differentiated chondrocytes using
von kossa stain, chondral calcification represented after induction of
chondrocyte differentiation on biocomposite membranes for 30 days
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