PEGylation of polymeric membrane surface via dopamine modification and click chemistry

• Main Authors: Jia-liang Zang, 臧家良
• Other Authors: Cheng-kang Lee
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/89275137757676051574

碩士 === 國立臺灣科技大學 === 化學工程系 === 98 === In this study, a facile method was developed to modify the surfaces of polymeric membranes with polyethylene glycol (PEG) to reduce the fouling of proteins and cells. Dopamine was first self-polymerized on membrane surface by oxidation via air exposure. Since the adherent polydopamine is strongly prone to couple with primary amine containing molecules, propargylamine was then coupled to the dopamine treated membrane surface. With alkyane groups exposed, the synthesized PEG-azide was easily grafted to the membrane surface via “click chemistry”. The existence of PEG on the membrane surface was confirmed by X-ray photoelectron spectroscopy; The surface morphology was studied by atomic force microscope. The antifouling capability of the PEGylated membrane was evaluated by incubating with bovine serum albumin solution (BSA) and growing fibroblast on top of it. 8.0e-02μmole PEG can be immobilized on 1cm2 membrane surface via evaluation of Nessler’s reagent.