| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 98 === In this research, the main purpose is the investigation of the effects of gold nanoparticles (GNPs) on behaviors of osteoblastic cells, including human gingival fibroblast cells (hGF) and rat osteosarcoma cells (UMR-106). The novel GNPs are prepared by using a novel process without any organic solvents (A and B types). Gold nanoparticles prepared by the commercial manufacturing process (M type) was are also used for the comparisons.
The results of TEM indicated that the shape and size of M and A types are similar. The B-type nanoparticles are with higher roughness and larger size, compaer with the other types. For the cells cultured without serum, A and B types are biocompatible while M type expresses the cytotoxicity. For the cells cultured with serum, A and M types significantly promote the cell activity, proliferation and early differentiation. On the other hand, B type is just biocompatible and ineffective, which may be resulted from the large size, high roughness, aggregation and low concentration of B-type GNPs in the culture medium. The effects of A and M type GNPs in this research would come from the protein adsorption on GNPs surfaces.
In conclusions, the present research reveals that the GNPs can enhance the activity, proliferation and differentiation of osteoblastic cells. Moreover, the GNPs prepared by using the novel process is more biocompatible than the convectional GNPs. As a result, the novel GNPs are with the excellent biocompatibility and the ability to promote activity, proliferation and differentiation of osteoblastic cells. The results in this study supported that the novel GNPs are highly potential in the biomedical applications.
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