Preparation and characterization of electrospun silk fibroin/TiO2 naofibers for biomedical applications

• Main Authors: Chi-Chuan Hsu, 徐其全
• Other Authors: Ming-Chien Yang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/81814696601350593607

碩士 === 國立臺灣科技大學 === 高分子系 === 98 === Using electrospinning to fabricate silk fibroin (SF) nanofiber film was investigated, thus blending different weight ratio of TiO2 with SF solution fabricates SF/TiO2 hybrid nanofibers with optimizative parameters. In this study, we investigated the preparation of nanofibers, and the physical properties, biocompatibility after blending with TiO2 nanoparticle. Scanning electron microscope (SEM) revealed that the SF nanofibers was formed with average diameters 386±73 nm when the concentration of SF was up to 10 %(w/v). The SF/TiO2 nanofibers film was also successfully prepared. FTIR identified the conversion of the random coil (Silk I) conformation of SF into β-sheet (Silk II) by treating with acetone. The maximum photocatalytic decomposition of methylene blue solution was nearly achieved to 50% after 24 hours. Compared with pure SF film, the thermal stability and surface roughness was improved by the blending with TiO2 nanoparticle. The SF/TiO2 nanofibers film provided a better level of equilibrium water content (EWC) and water vapor transmission rate (WVTR) than TegasorbTM (Hydrocolloid dressing). Complete blood count (CBC) and cytotoxicity showed no significantly difference. The SF/TiO2 nanofibers film was found to promote the adhesion, growth and spreading of L929 fibroblasts cells according to a three dimensional network of the nanofibrous structure, and exhibited antibacterial activities against Escherichia coli. In conclusion, the novel nanocomposite mats may be a good candidate as wound dressing for tissue engineering applications.