| Summary: | 碩士 === 國立臺灣大學 === 獸醫學研究所 === 98 === Naturally occurring avian reticuloendotheliosis virus (REV) infections is common in many domestic fowls. REV causes immunosupression, runting disease, and chronic lymphoma, resulting in a great economic loss. Although REV infection was common, no monitoring data were reported in Taiwan. Thus, the aims of this study were to investigate the REV infection in Taiwan Country chickens and to establish ELISAs for REV antibody detection. For REV monitoring in Taiwan Country chickens, 239 blood samples from a slaughter house and 170 blood samples from a breeder flock were tested for the presence of REV provirus by PCR. The results revealed that REV prevalence in Taiwan Country chicken flocks ranged from 75% to 100% in different kinds of chickens from a slaughterhouse. In the breeder flock, Nogoya breed had a prevalence of 11.1% (4/36) and S breed 38.5% (27/70). Two ELISAs were developed for REV antibody detection. One of the ELISAs was an indirect ELISA coating with recombinant protein p30 and the other was a blocking ELISA with expression env protein. The sensitivity and specificity of p30 ELISA were 92% (46/50) and 94.8% (91/96) and those of blocking ELISA were 90% (45/50) and 94.8% (91/96), respectively. Comparing with a commercial ELISA kit, the three ELISAs were correlated with neutralization result（p30 indirect ELISA 80.3％, env blocking ELISA 80.1％, and a commercial ELISA 80.5％). In conclusion, these two new ELISAs can be applied to detect anti-REV antibody in the field.
|
|---|
