Expression of human and Epstein-Barr virus-encoded microRNAs in Nasal Natural Killer/T-Cell Lymphoma

• Main Authors: Yi-Chun Lin, 林宜君
• Other Authors: 林中梧
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/44298249025946015758

碩士 === 臺灣大學 === 病理學研究所 === 98 === Nasal Natural Killer/T-Cell Lymphoma (NNKTL) is a distinctive type of extranodal lymphoma with a strong association with Epstein-Barr virus (EBV). MicroRNAs have been implicated in the development and biological behaviors of a variety of cancers. However, expression patterns of miRNAs in NNKTLs are still unknown. We screened the expression patterns of 39 EBV-encoded and 55 human microRNAs in NNKTL. Total RNAs were isolated from paraffin-embedded tissues of NNKTLs. Real-time quantitative PCR was used to analyze the expression levels of EBV and human microRNAs. We used three statistical methods to analyze our QPCR results. From the T Test results, we can know that EBV-miR BART13 * and Hsa miR-155, miR-210 and miR320 have significant difference between the early and late stage in NNKTL and from the repeated data also have same result. From the Cluster analysis results, we could not separate the early and late stage in NNKTL clearly base on miRNAs expression patterns.